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鼠尾膠原蛋白I型 澄清牛瘤胃液 PD1/PDL1 重組人 PD-1 頭孢吡普 重組人胰島素
Gene Name Synonym: CD340; ENV; ENVW; ERVWE1; HER-2; HER-2/neu; HER2; HERV-7q;HERV-W-ENV; HERV7Q; HERVW; HERVWENV; MLN 19; MLN19; NEU;NGL; TKR1
純度：> 90 % as determined by SDS-PAGE 內毒素：< 1.0 EU per μg of the protein as determined by the LAL method 表達宿主：HEK293 Cells 種屬：Human 預測 ：N 端Thr 23 分子量：The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, rh ErbB2 migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions. 生物活性 1. Measured by its binding ability in a functional ELISA. Immobilized human Erbb2 at 2.5 μg/ml (100 μl/well) can bind Herceptin with a linear range of 1.28-32 ng/ml. 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.2-1 μg/mL in the presence of 0.6 μg/mL Anti-ErbB2/Her2 Monoclonal Antibody. 3. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant (His Tag) (Cat: 10004-H08H) bound to Pertuzumab was 0.1 nM. 蛋白構建： A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus. 緩沖液： Lyophilized from sterile PBS, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. 運輸方式： In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. 穩定性 & 儲存條件： Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. 復溶： A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Anti-Mouse IgG (Fc Specific)-Peroxidase produced in goat, affinity isolated antibody adsorbed with human IgG
抗-小鼠IgG（Fc特異性）-過氧化物酶 山羊抗
goat
affinity isolated antibody
secondary antibodies
polyclonal
mouse
direct ELISA: 1:50,000 immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well
免疫球蛋白G（IgG）是一種糖蛋白抗體，其可調節免疫反應，如吞噬作用，也可參與自身免疫疾病的發展。小鼠IgG具有四種不同的同種型，即IgG1、IgG2a、IgG2b和IgG3。IgG1可調節小鼠的補體結合。 通過免疫特異性純化從山羊抗小鼠IgG抗血清中分離抗體，該過程可基本上去除所有山羊血清蛋白，包括免疫球蛋白（其不可與小鼠IgG的Fc片段特異性結合）。以0.2%戊二醛為交聯劑，將抗鼠IgG與過氧化物酶偶聯。該抗體制劑采用人IgG吸附固相制備，以確保組織或細胞制劑中的交叉反應性最低。
純化的小鼠IgG Fc片段
通過使用生物合成PND和鏈霉親和素涂層板進行ELISA試驗，從而對免疫小鼠血液中一種新的PND抗體的特異性進行檢測。HRP綴合的山羊抗小鼠IgG（Fc特異性）可用作二抗。
山羊抗小鼠IgG (Fc特定)-過氧化物酶抗體用于：
0.01M磷酸緩沖鹽緩沖液（pH7.4, 含有0.05% MIT）溶液。
通過Avrameas, S., et al., Scand.J. Immunol., 8, Suppl. 7, 7 (1978)等描述的兩步戊二醛方法進行制備。
Human PD-L1 / B7-H1 Protein, Mouse IgG1 Fc Tag, low endotoxin (MALS verified)
PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1
Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (PD1-H52A3) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).
Predicted N-terminus: Phe 19
This protein carries a mouse IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.4 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 0.1 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
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